The 50ml centrifuge tube with leakproof screw cap, with its unique thread structure design, plays an irreplaceable role in the key link of sample preparation. Faced with complex operations such as multiple centrifugation and violent shaking, the centrifuge tube uses precise mechanical bite and dynamic sealing mechanism to build a reliable protective barrier for key reagents such as lysate and washing solution, effectively avoid the risk of cross contamination, and provide a solid guarantee for the accuracy and reliability of gene sequencing experiments. The sample preparation of gene sequencing experiments, from cell lysis, nucleic acid extraction to purification and enrichment, requires multiple high-speed centrifugation and strong shaking in each step. In this process, the sealing structure of ordinary containers is very easy to loosen due to vibration, causing reagent leakage or sample cross contamination, which in turn interferes with subsequent gene fragment amplification and sequencing analysis. The thread structure of the 50ml leakproof screw cap centrifuge tube adopts an asymmetric tooth design, combined with micron-level precision CNC machining technology, so that the screw cap and the tube body are screwed together to form a multi-layer tight bite. Under the dual effects of centrifugal force and shock force, this structure will not only not loosen, but will produce a dynamic self-tightening effect due to the accumulation of friction torque between the inclined surfaces of the threads. When the centrifuge runs at a speed of thousands of revolutions per minute, the strong radial pressure generated by the liquid in the tube will cause the screw cap to fit further with the tube body, ensuring that the sealing pressure and external force are enhanced simultaneously, effectively resisting the problem of sealing failure caused by vibration. The advantages of leak-proof screw caps are particularly evident in the key step of lysing cells to obtain nucleic acids. Lysis solutions usually contain strong denaturants and proteinase K and other ingredients. If leakage occurs, it will not only corrode the experimental equipment, but also contaminate other samples, resulting in the failure of the entire experiment. The thread structure of the 50ml centrifuge tube significantly increases the resistance to liquid leakage through layers of nested physical barriers. The rounded corners of the thread crest and bottom eliminate stress concentration points, avoid thread damage caused by repeated tightening or external force impact, and ensure the long-term reliability of the sealing structure. Even in multiple consecutive high-speed centrifugation cycles, the fine threads can still maintain a tight bite to prevent the lysis solution from leaking out, ensuring the integrity of the sample and the safety of the experimental environment. The washing solution processing link also relies on the excellent leak-proof performance of the centrifuge tube. In the process of nucleic acid purification, washing solutions such as ethanol need to be centrifuged multiple times to remove impurities. These organic solvents are volatile and highly corrosive, and require extremely high sealing and chemical resistance of the container. The thread structure of the 50ml leak-proof screw cap centrifuge tube is combined with high-purity polypropylene (PP) material to show strong adaptability. The chemical stability of the PP material prevents it from swelling or embrittlement when it comes into contact with reagents such as ethanol, and the dynamic self-tightening characteristics of the thread structure ensure that during the centrifugation process, even if the pressure in the tube fluctuates due to temperature changes or liquid volatilization, the screw cap can automatically adjust the sealing strength to prevent leakage of the washing solution. This dual protection mechanism avoids the risk of sample contamination by residual washing solution and reduces the potential harm to operators and the laboratory environment caused by reagent leakage. The thread structure of the leak-proof screw cap continues to play an important role in sample transfer and temporary storage. Gene sequencing experiments often involve the collaboration of multiple operating platforms and experimenters. During the transportation of samples between different links, they may face bumps, tilts, etc. The multi-layer mechanical bite design of the 50ml centrifuge tube can effectively resist external force impact and ensure that the reagents in the tube are always in a sealed state. Even if the experimenter quickly removes or places the centrifuge tube in an emergency, the stability of the thread structure can prevent the lid from accidentally falling off, avoiding sample loss or contamination due to operational errors. The 50ml centrifuge tube with a leak-proof screw cap has successfully overcome the sealing problem in the preparation of gene sequencing samples by deeply combining the precise thread structure design with the needs of life science experiments. From complex centrifugal shaking operations, to the handling of volatile reagents, to the safe transportation of samples, its unique thread structure provides full protection for experimental samples with reliable mechanical bite and dynamic sealing performance.